Isolation and Selection of Fungi that Produces Lignocellulolytic Enzymes Isolated in Sugarcane Bagasse Rahmad(1*) and Nurmiaty(1)
1) Departement of Estate Crops Cultivation, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia
Abstract
Lignocellulolytic enzymes are produced by fungi that occur in sugarcane bagasse. The aim of this research is to isolate and to identify the fungal isolates that produce lignocellulolytic enzymes in sugarcane bagasse from Takalar Sugar Factory, South Sulawesi. The fungal isolates are isolated, purified, and selected for their ability to produce lignocellulolytic enzymes. Testing of lignocellulolytic enzymes consists of cellulase enzyme testing using PDA (Potato Dextrose Agar) media, Czapeck (difcoTM) enriched with CMC, hemicellulase enzymes enriched with xylan, and clear zone testing with iodine solution and ligninase enzyme testing using MEA Malt Extract Agar (MEA) enriched media, xylan-enriched hemicellulase enzymes, clear zone testing with iodine solution, and ligninase enzyme testing using Malt Extract Agar (MEA) enriched media with tannic acid. As many as 23 fungal isolates are successfully isolated, and most of the fungi obtained consists of 10 fungal isolates Aspergillus sp., 4 isolates of Penicillium sp. and 5 isolates of Trichoderma sp. There are 17 fungal isolates producing cellulase enzyme, 19 isolates producing hemicellulase enzyme, and 13 isolates producing ligninase enzyme. Fungal isolate of Amblyosporium sp. produces the highest cellulase and ligninase enzymes, and fungal isolate of Aspergillus sp8 produces the highest hemicellulase enzyme. The results of this study show that there is a fungal isolate derived from sugarcane bagasse that has a high ability to produce lignocellulolytic enzymes.
