The identification of ethanol and aromatic compounds production from delignification of Cacao pod husk using Phlebia sp.MG-60 Irnia Nurika; Zuliyan Agus Nur Muchlis Majid; Suprayogi
Department of Agroindustrial Technology, Faculty of Agricultural Technology,
Universitas Brawijaya
Abstract
Biological pre-treatment of lignocellulose biomass from cacao pod husk by white rot fungus Phlebia sp.MG-60 can represent the integrated biorefinery concept. This agricultural residue can be effectively transformed to both renewable energy and high value added chemicals. The objective of this study was to identify the potential of white rot Phlebia sp. MG-60 to produce ethanol and aromatic compounds under consolidated biological processing (CBP). By adding the inducer (MnSO4), the results revealed that the addition of MnSO4 could escalate the activity of manganese peroxidase from Phlebia sp. MG-60 in order to depolymerize lignocellulose. Using LCMS-MS several aromatic compounds such as 2,3-Dimethylphenol, trans-Cinamic acid, caffeic acid, and vanillin were obtained from cacao pod husk incubated by the fungus for 28 days. Furthermore the amount of ethanol produced during the bioconversion process was 2g/L. Further studies are required to determine factors influencing optimal ethanol production.
