Microbial biomass and phospolipid fatty acid (PLFA) profile changes in bioconversion of lignocellulose biomass from wheat straw. Irnia Nurika
Agroindustrial Department, Faculty of Agricultural Technology, Universitas Brawijaya
Abstract
In conjunction with fungal biomass measurements, Serpula lacrymans was shown to grow very well on wheat straw solid state cultivation. This study aimed to determine the relative composition of PLFA during fungal growth. The PLFA analysis was carried out on the upper layer of the solvent extraction and was performed on the incubation series for up to 35 days. Relative abundant (%) of each fatty acid methyl ester (FAME) produced during the fungal growth was measured by comparing the amount of an individual FAME with total FAME detected. Meanwhile the fungal biomass was measured using ergosterol method and by using conversion factor the fungal biomass in wheat straw culture was calculated. The dominant PLFA released by the fungi were: 24:1n9 (nervonic acid), 18:1n9c (oleic acid), 16:0 (palmitic acid), and 18:2n6c (linoleic acid). The amount of PLFA produced on wheat straw solid-state cultivation correlated with the fungal biomass. This evidence showed that S.lacrymans could be suggested as the promising brown rot fungi, which is well suited to the bioconversion of wheat straw which required minimal external inputs.
