Bringing UV/Vis Spectroscopy Sensing into Portable Device for Lard Adulteration Detection Braja Manggala1, Tiara Murni Simaremare1, Musthofa Lutfi1, Ary Mustofa Ahmad1, Muhammad Arif Kamal2, Freini Dessi3, Zaqlul Iqbal1*
1Department of Agricultural Engineering, Universitas Brawijaya, Malang, Indonesia
2Department of Agro-industrial Technology, Universitas Brawijaya, Malang, Indonesia
3Department of Biotechnology, Universitas Brawijaya, Malang, Indonesia
Abstract
Developing fast detection system on lard adulteration is a challenging attempt for moeslem populated countries including Indonesia. And recently, only bench-top based detection method such as Polymerase Chain Reaction (PCR) could provide remarkable result for measuring the adulteration. However, the method requires lengthy procedure and high cost which is not suitable for small-medium scale culinary businesses for in-situ measurement purpose. So therefore, this research aims to identify the feasibility of small UV/Vis spectroscopy based sensor to predict lard adulteration on cooking oil. In order to reach the conclusion several stages had been done: (1) Designing 3D-printed portable sensing device; (2) creating quantitative and qualitative calibration model on lard adulteration samples using The unscramble XI with three different measuring distance: 0.2mm, 0.6mm and 0.8mm from cuvette sample; (3) validating the model with bench-top instrumentation. The best quantitative result using Principal Component Analysis (PCA) provided by 0.8mm distance with 0.88, 0.85 and 4.2 for R2 calibration, R2 validation and error respectively. As for the best quantitative result using Partial Least Square (PLS), was obtained also from 0.8 mm distance which accounted for 0,89, 0,87 and 3,3 for R2 calibration, R2 validation and error respectively. These were very promising result compared with bench-top version of UV/Vis spectrophotometer. And for the future research, the accuracy of measurement need to be achieved by lowering lard concentration on sample.
Keywords: Lard oil, fast detection system, portable device, UV/Vis spectroscopy.
