Rapid Detection of Porcine DNA by Real Time Polymerase Chain Reaction in Imported Instant Noodles Agustin Krisna Wardani, Aji Sutrisno, and Aisyi Sakina Rifani
Department of Food Science & Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang
Abstract
Indonesia is the worlds second largest instant noodle market, accounting for almost 15% of all instant noodle consumption on earth. Each year, about 13 billion packets of instant noodles are sold in Indonesia. To ensure that no traces of porcine DNA in imported instant noodles products, an effective detection method is required for consumer safety and protection especially in Indonesia, the worlds largest Muslim population. In this study, a mitochondrial D-loop 443 primer was used to identify the presence of porcine DNA in ten samples of instant noodles by Real-Time Quantitative Polymerase Chain Reaction (qPCR). Ten samples of imported instant noodles were purchased from local minimarket in Malang, Indonesia. The porcine DNA was amplified at annealing temperature of 53 oC for 39 cycles. The result confirmed that three samples of instant noodles were found to contain porcine DNA. This method could be an effective and simple method to authenticate Halal products in processed foods which would verify food labeling and provide consumer protection.
