THE GROWTH OF PAPRIKA (Capsicum annuum L.) FROM COTYLEDON ON VARIOUS MEDIA IN VITRO Hawalina Kasim1, Yusran1, Hastuti1, Jeki1, M. Anshar1 and Saidah Labalado2
1 Faculty of Agriculture, Tadulako University, Jl. Soekarno Hatta Km. 9 Palu,Central Sulawesi, 94119.
2 Research Center for Horticultural and Estate Crops, National Research and Innovation Agency Republic of Indonesia, Jl. Raya Jakarta-Bogor Km.46 Cibinong, West Java, Indonesia, 16911.
Abstract
This research aimed to determine the growth of paprika (Capsicum annuum L.) from cotyledon on various media in vitro. The research was arranged as completely randomized design with 10 treatments, four replications, used MS fortified with: 1). 3 benzylamino purine (BAP) + 0.3 indoleacetic acid (IAA) + 0.2 silver nitrate (AgNO3). 2). 3 BAP + 0.3 IAA + 0.3 gibberellin (GA3) + 0.2 AgNO3. 3). 3 BAP + 0.3 GA3 + 0.3 naphthaleneacetic acid (NAA) + 0.2 AgNO3. 4). 3 BAP + 1.5 NAA + 3 AgNO3 + 1 glutamine. 5). 3 BAP + 0.5 NAA + 3 AgNO3 + 1 glutamine. 6). 4 BAP + 1.5 NAA + 3.5 AgNO3 + 1 glutamine. 7). 5 BAP + 1 IAA. 8). 10 BAP + 2 GA3. 9). 5 thidiazuron + 2 GA3. 10). 7.5 kinetin + 2 GA3. All units are in mg/L. The treatment effects were observed on callus and shoot growth, analyzed by ANOVA and the mean differences were tested using Tukey Test. All treatments induced callus but only 7), 9) and 10) regenerated shoots of 1,38, 1.50 and 3.75 successivelly. Addition of 7.5 mg/L kinetin + 2 mg/L GA3 was the best to induce shoots.
