GC-MS Metabolomic Profiling of Garcinia intermedia Leaf with Several Sample Drying Methods Tri Budiyanti, Winda Nawfetrias, Sri Hadiati, Riska, Jumjunidang, NLP. Indriyani, Ellina Mansyah, R Reninta and ED Husada
Research Center for Horticultural and Crops, Cibinong Science Center, National Research and Innovation Agency (BRIN),
Research Centre for Genetic Engineering, National Research and Innovation Agency (BRIN),
Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN).
Abstract
Garcinia intermedia, commonly called cherry mangosteen or lemon drop mangosteen, originates from Mexico and Central America. This plant is not cultivated in Indonesia because the fruit is less tasty than mangosteen (Garcinia mangoestana). Information about the metabolome content in Garcinia intermedia needs to be known to increase its commercial use. Several studies have shown that this species has anti-microbial content that benefits health. This study aims to perform metabolomics profiling in the leaves of Garcinia intermedia using 3 (three) treatments of leaf drying methods: freeze drying, oven, and wind drying via Gas Chromatography-Mass Spectrophotometry (GC-MS). The study^s results identified 60 volatile compounds found in the leaves of Garcinia intermedia. Samples dried using the Freeze Drying method and drying at room temperature (wind dried) could identify 46 compounds, while samples dried by the oven could identify 32 compounds. The results also showed volatile compounds at the highest peaks from the GC-MS analysis, namely Caryophyllene, Gamma Muurolene, Naphthalene, Octadecane, delta.-Tocopherol, and Friedelan-3-one. These compounds are helpful for antioxidants, anti-inflammatories, and vitamin E, which can benefit health.
