Aflatoxins Contamination in Duck Feedstuff Collected from Indramayu District Y Anastasia, R Maryam, R Widiastuti, Dalilah
Research Center for Veterinary Science
Abstract
Aflatoxins are secondary metabolites produced by Aspergillus fungi. Feed exposure to aflatoxins may cause a decreased immune system and low productivity outcomes in poultry. This study conducted a limited survey to determine aflatoxin contaminations in feedstuff. 34 feed samples were analyzed to determine aflatoxin B1, aflatoxin B2, aflatoxin G1, and aflatoxin G2 using High-Performance Liquid Chromatography (HPLC) fluorescent detector. Aflatoxins were extracted from feed samples using acetonitrile : water (84:16,v/v), purified with a solid phase extraction (SPE) column, and detected through a fluorescent detector at wavelengths 365 nm (excitation) and 450 nm (emission). The results show a high concentration of AFB1 contamination in 34 samples. AFB1 were detected in 28 samples ranging from 1.38 to 1467,37 ng/g. AFB2 were detected in 27 samples ranging from 0.12 to 6.86 ng/g for AFB2. On the other hand, AFG1 and AFG2 were not detected in all samples. The high prevalence and concentration of AFB1 in most samples were harmful to the animals and should be a concern to prevent any adverse effect on public health
