Ochratoxin A (OTA) Contamination Detection by Enzyme-Linked Immunosorbent Assay (ELISA) Prima Mei Widiyanti and Romsyah Maryam
National Research and Innovation Agency, Indonesia.
Abstract
Indonesia is a country with a tropical climate with high temperatures, rainfall, and humidity, which are conducive to the growth of fungi in various agricultural commodities, food and feed. Ochratoxin A (OTA) is the result of secondary metabolites from the toxigenic fungi of the genus Aspergillus and Penicillium. OTA is carcinogenic, nephrotoxic, neurotoxic, and it can cause health problems. OTA can be detected by an enzyme-linked immunosorbent assay (ELISA). The ELISA method has more advantages than other methods, because it can detect samples quickly, easily, economically, specifically, and sensitively. The purpose of this study was to detect the presence of ochratoxin A (OTA) in samples by ELISA. The results of this study showed that OTA contamination in 50 samples were 92%, with an average value of 6.18 ppb. The levels of OTA contamination in samples were below the regulation.
