Effectiveness of Media B5 (Gamborg) for Multiplication of Sengon (Paraserianthes falcataria L. Nielsen) in vitro Gusmiaty, Syamsinar , Muh. Restu, Iswanto, Nur Aida, Siti Halimah Larekeng
Faculty of Forestry, hasanuddin University
Abstract
Paraserianthes falcataria (L.) Nielsen, known as sengon, is one of the most important versatile pioneer species in Indonesia. The Sengon plant is one of the fastest-growing plants in the tropics. The purpose of this study was to obtain the best media at the multiplication stage of sengon with the addition of ZPT cytokinin BAP and kinetin either singly or in combination. The research design used was a completely randomized design (CRD). The results of the study, which were the best media on sengon plantlets, were Media M2 (B5 + BAP 0.1) which was the medium with the highest number of shoots. Media (B5 + BAP 0.2) was the fastest medium for leaf emergence at 1 MST. The result of the Poisson test analysis on M2 media (B5 + BAP 0.1) is a medium with a value that has a very significant effect on the number of leaves compared to other media. The average height of the highest sengon plantlets was M1 media (B5 Control), with a value of 1.73 cm. The highest percentage of dead plantlets on M4 media was 33% and on M9 media was 17% which was characterized by browning, which led to cell death. Contaminated plantlets were only shown on media M4 (BAP 0.2), M5 (Kinetin 0.2), and M9 (BAP (0.2) + Kin (0.2) caused by bacteria with a percentage of up to 17%, which caused plantlet growth inhibition.
Keywords: multiplication, in vitro, Medium, Paraserianthes falcataria
