DIVERSITY OF SINGLE NUCLEOTiDE POLYMORPHISM (SNP) GEN FREM2 IN BALI CATTLE Eva Amalia Pertiwi (1), Jakaria (2*), Mokhamad Fakhrul Ulum (3),
(1) Magister Program in Animal Science
Faculty of Animal Science, IPB University (Bogor Agricultural University)
(2*) Department of Animal Production and Technology, Faculty of Animal Science,
IPB University (Bogor Agricultural University), Bogor 16680, Indonesia
(3) Department of Reproductive Clinics and Pathology, Faculty of Veterinary Medicine,
IPB University (Bogor Agricultural University), Bogor 16680, Indonesia
Abstract
FREM2 gene is one of the genes that is suspected to be strongly associated with meat quality. This gene is intensively explored in Hanwoo cattle, whereas in Bali cattle is never reported. This study aimed to identify the diversity of SNP FREM2 gene in Bali cattle with a direct sequencing method. The total sample used was 20 individuals consisting of 10 individuals from the slaughterhouse in Banjarmasin and 10 individuals from the Bali cattle Breeding Center in Bali Province, Indonesia. The sequence data of FREM2 gene exon 6 introns 6 was analyzed by using the MEGA10 program to find SNP and FREM2 gene diversity. Popgen 3.2 was used to analyze the genotype and allele frequencies. The results of this study showed that FREM2 gene has 4 SNP namely SNP G>A at g.89327, SNP C>A at g.89463, SNP C>T at g.89573, and SNP C>T at g.89633. The findings of snp identified that frem2 is polymorphic. The allele frequencies of each SNP were G= 0.325 A= 0.675, C= 0.325 A= 0.675, C=0.325 T=0,675, and C= 0.800 T= 0.200, respectively. Based on the results, it can be concluded that FREM2 gene exon 6 and introns 6 were polymorphic,
Keywords: Bali cattle, FREM2 gene, SNP
Topic: Animal Genetics, Breeding and Livestock Production
