DETERMINED OF SINGLE NUCLEOTIDE POLYMORPHISM (SNP) MAOA GENE IN BEEF CATTLE Nadira Andarini Siregar (a), Ronny Rachman Noor (b), Jakaria (b)
(a) Magister Program in Animal Science
Faculty of Animal Science, IPB University (Bogor Agricultural University)
(b) Department of Animal Production and Technology, Faculty of Animal Science
IPB University (Bogor Agricultural University), Bogor 16680, Indonesia
Abstract
The MAOA genes was responsible in aggressive behavior in beef cattle. This study aimed to identify the diversity of SNP MAOA gene in beef cattle with a direct sequencing method. DNA sample were collected from 41 cattle, including Bali (30 indivduals), Madura (6 individuals), and PO (5 individuals). Polymorphism of MAOA gene was analyzed using PCR with forward and reverse primers that amplified the exon 1 fragment of the maoa gene and direct sequencing methods. The sequence data of MAOA gene was analyzed by using the MEGAX program to find SNP MAOA gene. Popgene 3.2 was used to analyze the genotype and allele frequencies, observation (Ho), expected (He) heterozygosity, and Hardy-Weinberg equilibrium test . The results of this study showed that MAOA gene has 2 SNP namely SNP c.828G>A and SNP c.849G>A. The allele frequencies of each SNP were G= 0.833 A= 0.1667 and G= 0.667 A= 0.3333 respectively. Results of the SNP c.828G>A and SNP g.849G>A were polymorphic in Bali cattle. In Madura and PO cattle were monomorphic. The values of Ho and He in SNP c.828G>A were 0.333 and 0.2825 in Hardy-Weinberg equilibrium (P>0.05) and the values of Ho and He in SNP c.849G>A were 0.666 and 0.452 in Hardy-Weinberg non equilibrium (P>0.05). In Bali cattle, two novel SNP candidates were found in position of c.828G>A and g.849G>A which were potential to be used as genetic markers of aggressive behaviour. As result this study, it can be concluded that gene exon 1 was polymorphic.
Keywords: Beef cattle, MAOA gene, SNP
Topic: Animal Genetics, Breeding and Livestock Production
