Local Arbuscular Mycorrhizal Fungi Diversity in Rubber Plants (Hevea brazilian) in Bulukumba with an Agroforestry Pattern Gusmiaty (a*), Nur Padli (a), Muh. Restu (a), Mukrimin (a), Dian Sasmita (a), Syamsuddin Millang (b), Tutik Kuswinanti (c), Muh. Akhsan Akib (d), Syatrawati (e) and Retno Prayudyaningsih (f)
(a) Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Universitas Hasanuddin, Makassar Indonesia *gusmiaty[at]unhas.ac.id
(b) Silviculture and Tree Physiology Laboratory, Faculty of Forestry, Universitas Hasanuddin, Makassar Indonesia
(c) Departement of Pest and Plant Disease, Faculty of Agriculture, Hasanuddin University, Makassar Indonesia
(d) Faculty of Agriculture, Animal Husbandry, and Fisheries, Universitas Muhammadiyah Parepare, Parepare, South Sulawesi, Indonesia
(e) Food Crop Production Technology, Pangkep State Polytechnic of Agriculture, Pangkep, South Sulawesi, Indonesia
(f) Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Jawa Barat, Indonesia
Abstract
This research provides a better understanding of the various types of Arbuscular Mycorrhizal Fungi (AMF) associated with agroforestry pattern rubber plantations. By knowing these types, the most suitable AMF species can be processed and selected to increase the productivity of rubber plants. This study aims to explore, identify, and inventory AMF in the rhizosphere of rubber plantations in agroforestry patterns. This research was conducted in March 2023. Soil sampling was carried out in Jojjolo Village, Ballasaraja Village, Bulukumpa District, Bulukumba Regency. Sampling of soil and roots using the method International Centre Research in Agroforestry (ICRAF). Then for the extraction of spores using the wet filter pouring technique and centrifugation technique. The filter results that have been transferred to the petri dish. Spores were observed and counted under a dissecting microscope with 4x magnification. Then transfer the spores based on their morphotype onto filter paper with vertical and horizontal lines in a 0.5 cm x 0.5 cm cup to facilitate observation. The results showed 9 spore morphotypes at 3 density levels. Among them, at rare densities, 954 spores were found with an average of 64 spores per plot. Then at medium density, 952 spores were obtained with an average of 63 spores per plot, and at a dense density, 766 spores were obtained with an average of 51 spores per plot.
