Antimicrobial potency of catfish (Clarias gariepinus) mucus Tetri Widiyani, Ririn Dwi Astuti, Ranita Restu Nur Azizah, Shanti Listyawati, Tjahjadi Purwoko, Agung Budiharjo, Ratna Setyaningsih
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret
Jl. Ir. Sutami 36 A Surakarta 57126, Indonesia.
tetriwidiyani[at]staff.uns.ac.id
Abstract
Catfish mucus is secreted by epidermal tissue which function as a defense tool against parasites. Fish mucus contains various antimicrobial substances. The purpose of this study was to determine the antibacterial and antifungal activities of catfish (Clarias gariepinus) mucilage extracts on Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa ATCC 27853 and Aspergillus flavus as well determine the protein profile. The catfish mucus sample was extracted in glacial acetic acid solvent. Antimicrobial testing was performed in vitro by using disc diffusion method to determine inhibitory activity on microbials growth. Protein profile contained in the mucus was electrophored by using SDS-PAGE method. Antimicrobial test showed that catfish mucus perform inhibition zone against MRSA and P. aeruginosa ATCC 27853 but not against A. flavus. Aspergilus flavus was not inhibited by catfish mucus extract because fungi is known has a relatively high survival rate. Electrophoresis results 4 protein bands with molecular weight (MW) respectively 117.56, 58.82, 25.37, and 15.47 kDa. Protein band 15.47 kDa is rather similar to lysozyme (MW 15 kDa), a protein which is capable of eliciting microbial activity. Nevertheless it indicated the presence of low level protein contents. Therefore the antimicrobial activities relatively low.
