Optimization of Silver Nanoparticle Synthesis Using Fingerroot (Curcuma rotunda L.) Rhizome Extract as a Bioreduction agent Wisnu Widikdo, Purwantiningsih Sugita*, Mohammad Khotib
Departement of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia
Abstract
Curcuma rhizomes are reported to have high total phenolic content. Phenol compounds can act as a bio reductor in the reduction of metal into metal nanoparticles. Five Curcuma rhizome extract were chosen for bio reductor, such as temu putih (Curcuma zedoaria), temu hitam (Curcuma aeruginosa), temu kunci (Curcuma rotunda L.), temu giring (Curcuma heyneana), and temu mangga (Curcuma amada). The silver nanoparticles (AgNPs) were synthesized by mixing 0.2 g of rhizome extract with AgNO3 then, the solution irradiated with a 366 nm UV lamp at a particular time and pH. The synthesis of AgNPs was confirmed by the colour change of the solution from yellow to dark brown and by UV-Visible spectroscopy. Based on UV-Vis spectra, the highest silver nanoparticle yield is obtained by using temu kunci extract as a bio reduction agent. Their optimum conditions were analyzed using response surface methodology (RSM) based Box-Behnken design (BBD) using Minitab 17. The key factors are pH, irradiation time, and mass ratio of AgNO3. Statistics results revealed that 0.13 g of silver nitrate with 178 minutes of irradiation time at pH 11.5 were the optimum values for AgNPs biosynthesis. The characterization of AgNPs is not yet finished.
Keywords: Box-Behken Design, Curcuma, C. rotunda, silver nanoparticles.
