Quality of spermatozoa of tiger shrimp (Penaeus monodon) fed with of blood cockle (Anadara granosa). Dody DharmawanTrijuno1*, Andi Aliah Hidayani1, Muhammad Fatratullah Muhsin1, Rosmiati2
1. Fisheries Department, Faculty of Marine Science and Fisheries, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Tamalanrea, Makassar, South Sulawesi Indonesia.
2. Research Institute for Brackishwater Aquaculture and Fisheries Extension, Jl. Makmur Dg. Sitakka No. 129, Maros 90512, South Sulawesi, Indonesia.
Abstract
One of the factors of tiger shrimp Penaeus monodon low level natural mating in fishpond and controlled tank is suspected due to the low quality of male broodstock spermatozoa. This study aims to determine the best concentration of blood cockle Anadara granosa to increase the quality of spermatozoa of tiger shrimp broodstock from fishpond. Twenty one days of feeding experiment was carried out with three types of feed, that were pellet feed (100%), combination of blood cockle (50%) and pellet feed (50%), and feeding of blood cockle (100%). Twenty-seven male tiger shrimps were used with weight range of 53-78 g. Spermatophores were sampled at the base of the 5th swimming leg and the spermatozoa was excreted, then diluted using physiological NaCl solution, and they were observed and counted using a haemocytometer. The number of spermatozoa density and the percentage of abnormal spermatozoa were analyzed using One Way ANOVA test and continued with the LSD test, while the spermatophore weight was analyzed by non-parametric data analysis using the kruskal-wallis test, and the spermatozoa morphology was presented descriptively. In the feeding treatment of 100% pellet, the mean density of spermatozoa was 27,03x108 cells/ml with an abnormality of 36.05%. In the feeding treatment of 50% blood cockle and 50% pellet, the mean density of spermatozoa was 39,69x108 cells/ml with an abnormality of 28,70%. While in the feeding treatment of 100% blood cockle the mean density of spermatozoa was 63,75x108 cells/ml with the percentage of abnormalities was 25%. It was concluded that feeding the shrimp with 100% of blood cockle was the best feed in increasing the quality of spermatozoa- increased spermatozoa density and decreased abnormality of spermatozoa.
