Isolation and Characterization of Thermophilic Bacteria from Waepella Hot Spring for Amylase Production Indrayani Indrayani1*, Subari Yanto1, Amiruddin Hambali1, Ardiansyah Ardiansyah2
1Study Program of Agricultural Technology Education, Faculty of Engineering,
Universitas Negeri Makassar, South Sulawesi, Indonesia
2Aquaculture Department, Agricultural Polytechnic State of Pangkep, Makassar-ParePare Km. 83,
South Sulawesi, Indonesia
Corresponding author: indrayani[at]unm.ac.id
Abstract
Bacteria are group of organisms commonly found in extreme habitats including hot springs. Bacteria that live in extreme habitats have the potential to produce commercial products, including the amylase enzyme which is widely used in the food and beverage industries. This study aims to obtain isolates of thermophilic bacteria from Waepella hot springs in Sinjai Regency, South Sulawesi which have the potential to be developed as amylase enzyme producer. Water samples were taken at 3 points with temperatures of 49, 53 and 550C, respectively. Bacterial isolation was carried out using the agar plating technique on TSA media. Pure bacterial isolates were obtained by repeated streaking on TSA media. The isolates obtained were then characterized by morphology, gram staining, catalase test, endospore test and amylolytic test. There are 20 isolates successfully obtained, 9 of which have the ability to produce amylase enzymes. ISO SS 55 isolate was the isolate with the highest amylolytic activity with a clear zone diameter of 23 mm. This isolate has the potential to be developed as a producer of amylase enzymes.
Keywords: extremophile bacteria, hot springs, amylolytic activity
