Sargassum aquifolium juice prevents raised cholesterol levels and muscle histopathological damage in rats fed an atherogenic diet Darmawan Jalu Prakoso and Muhamad Firdaus
Fishery Products Technology Study Program, Faculty of Fisheries and Marine Science Universitas Brawijaya.
Abstract
Hypercholesterolemia is a condition in which cholesterol levels in the body exceed normal limits. Hypercholeterolemia has many factors such as lifestyle and consuming foods that are high in fat. The number of natural ingredients to prevent the increase of cholesterol levels in the body. Sargassum aquifolium is one of the natural ingredients that is able to prevent the increase in cholesterol levels which contains its bioactive compounds. S. aquifolium can be extracted by slow juicy method to remove bioactive compounds. This study aims to determine the benefits of giving S. aquifolium juice to prevent the increase in cholesterol levels in the body and damage to the muscle profile of rats. The study used S. aquifolium seaweed taken from the coastal bay of Ekas Buana, West Lombok, Lombok, West Nusa Tenggara. Wistar rats (Rattus novergicus) aged 2 to 3 months, weighing approximately 150 g as experimental animals. Modeling of hypercholesterolemia was carried out through atherogenic feeding for 6 months. The phytochemical method was carried out based on the Harborne method. This study consisted of 5 treatments, namely, negative control treatment, positive treatment, treatment of atherogenic diet feeding by giving S. aquifolium juice 1x, 2x and 3x a day. Parameters observed were cholesterol levels and muscle profiles. Statistical analysis used is one way ANOVA and Kruskall Wallis test. The results showed that the administration of an atherogenic diet was able to increase cholesterol levels and damage muscle profiles. Bioactive compounds from the phytochemical test of S. aquifolium juice in the form of saponins, tannins and steroids that are capable of being antihypercholesterolemic by giving 2 times a day. The conclusion is that S. aquifolium juice has bioactive compounds that can prevent the increase of cholesterol levels and prevent damage to the muscle profile of rats when given 2 times a day, but are toxic when given 3 times a day. The conclusion is that S. aquifolium
