The Mortality Rate of Tiger Shrimp Penaeus monodon Injected with White Spot Syndrome Virus (WSSV) Suspension Challenged with Mangrove S. alba Extract at different concentrations Muliani1, Ince Ayu Khairana kadriah1, Bunga Rante Tampangallo1, and Muharijadi Atmomarsono1
Research Institute for Brackishwater Aquaculture and Fisheries Extension
Abstract
ABSTRACT. This study aimed to examine the effect of different concentrations of mangrove leaf S. alba extract on the mortality rate of tiger shrimp P. monodon. The plastic container filled with 30 L sterile seawater salinity 30 ppt stocked with 10 pcs of tiger shrimp 15 g in size. Six treatments were tested- A). 0.01%- B). 0.1%- C). 1%- D). 10%- E). 100%, F). control (shrimp injected with WSSV suspension without mangrove extract). Each treatment with three replication. The WSSV suspension was mixed with mangrove extract (from boiling 1 kg in 5 L of water cooked to 2.5 L of fresh leaves of S. alba) according to the treatment with a ratio of 1:2 and incubated at a temperature of 29 oC for 3 hours. Then the mixture is injected into tiger shrimp by intramuscular as much as 100 μ-L/shrimp. Tiger shrimp mortality was observed daily, while total hemocytes, ProPO, and differential hemocytes were observed at the end of the study. The results showed that on the first day, the mortality of tiger shrimp was highest in treatment D and the lowest in treatments A and F(control), however, from the second day until the seventh day (the end of the study), mortality of tiger shrimp was highest at the treatment A and lowest on treatment E. The results of this study showed that the concentration of boiling extract of S. alba below 10% is not effective in inactivating WSSV, therefore the mortality of tiger shrimp is still high.
