Clinical Symptoms Of Rabbitfish, Siganus Guttatus, Which Died While Cultivated In Ponds And Floating Net Cage B.R. Tampangallo, *Asda Laining, *Kamaruddin, *Muharijadi Atmomarsono, dan *Muliani
Research institute for Brackish Aquaculture and Fisheries Extension
Abstract
Rabbitfish is an economical fish that can be cultivated. Fish farming is inseparable from diseases. The study aimed to observe the clinical symptoms of dead rabbitfish in ponds and floating net cages. The research method is a survey by looking at the behavior of fish in the cage/aquarium, external physical abnormalities, and abnormalities of the internal organs. Isolate bacteria from the liver, kidneys, intestines, and gills using TSA, TCBA, and BHIA plate media. In this study, an analysis of the presence of the VNN virus was also carried out using the PCR method. Confirmation of the pathogenicity of the successfully isolated isolate is carried out in a controlled container. The results showed that the fish cultivated had no appetite, less active movement, and finally swam undirected and then died. Clinical symptoms of dead fish are blurry to white eyes, pale gills, pale liver to yellow and hemorrhage, empty intestines to hemorrhage, swollen lymph, and kidneys dilated to hemorrhagic. PCR results showed that the fish were negatively infected with VNN. The colonies of bacteria growing in the TCBSA plate medium are round, convex, slippery edges, and yellow in color. Blasting results showed this isolate was Vibrio alginolitycus. The bacterial colonies grown in the dominant TSA plate medium are round, convex, and yellow in color and the colonies in the BHIA medium are round, convex, and yellowish beige in color. Isolates of V. alginolitycus and colonies of BHIA media infected in Baronang Fish do not cause fish to die, but in fish injected with isolates from BHIA suspected of streptococcus, showing physical abnormalities in the form of transparent white bumps/bubbles on the front of the head. In fish injected with Streptococcus agalactia IBN2M, bacteria cause the fish to die up to 100% on the 12th day after injection.
Keywords: Rabbitfish Cultivation, feed, hemorrhagic, and bacteria
