CHARACTERIZATION OF ALFA GLUCOSIDASE INHIBITOR STREPTOMYCES SP. IPBCC.A.29.1556 AQUEOUS EXTRACT: AN ENDOPHYTE OF INDONESIAN FICUS DELTOIDEA Isra Janatiningrum(a), Yulin Lestari(b,c), Dedy D. Solihin(b), and Anja Meryandini(b)
a) Pharmacy Study Program, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta
b) Department of Biology, Faculty of Mathematics and Natural Sciences, Institut Pertanian Bogor.
c) Tropical Biopharmaca Research Center, Bogor Agricultural University
Abstract
Filamentous bacteria have been known as actinobacteria which have the ability to produce various secondary metabolites including α--glucosidase inhibitor. The α--glucosidase inhibitor has been identified to be potentially valuable for the treatment of diabetes mellitus. Endophytic actinobacteria have the ability to produce bioactive compounds that are similar to their hosts. Indonesian Ficus deltoidea is one of medicinal plants which has the activity of α--glucosidase inhibitor. The α--glucosidase inhibitor have been characterized by optimizing compound production, fractionation, analysis using TLC and LC-MS, and identifying inhibitor mechanisms. The α--glucosidase inhibitor substance is present in Streptomyces sp. IPBCC.a.29.1556 aqueous extract. Aqueous extract was separated and Fraction 1 had IC50 value of 58.8 μ-g/ml, less than acarbose (IC50 = 90.4 μ-g/ml). So, it can be concluded that the Streptomyces sp. IPBCC.a.29.1556 aqueous extract activity is better than acarbose. Kinetic studies revealed that these fraction inhibited the enzyme non-competitively. Chemical profile based on LC-MS, fraction 1 showed the presence of Phenylpropynal, Butyric acid, Solketal, and p-Coumaric acid which might contribute individually or synergistically to the observed α--glucosidase inhibitor activity. These results suggest that Fraction 1 from aqueous extract of Streptomyces sp. IPBCC.a.29.1556 is potential to produce α--glucosidase inhibitor for the management of postprandial hyperglycemia.
