STANDARDIZATION OF SIMPLICIA, EXTRACT AND BIOACTIVITY TEST OF EXTRACT AND FRACTION OF RUKAM LEAVES (Flacourtia rukam Zoll. & Moritzi) apt, Sri Hainil,M.Farm.,Prof. Dr. apt. Dachriyanus., Lisa Fernandez
Institut Kesehatan Mitra Bunda
Abstract
Rukam (Flacourtia rukam Zoll. & Moritzi) is a native Indonesian plant that has the potential to be a medicinal ingredient. Rukam is also found in China, India and Southeast Asia. Previous studies have shown that the leaves of the rukam plant have moderate antioxidant activity. The purpose of this study was to determine the antibacterial, antifungal, antioxidant activity and toxicity effects of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of rukam leaves. Antibacterial activity test against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 and antifungal activity test against Candida albicans ATCC 10231 using the disc diffusion method. The antioxidant activity test used the DPPH (2,2-diphenyl-1-picrylhydarzyl) method and the toxic activity test used the BSLT (Brine Shrimp Lethality Test) method. The results showed that the ethanol extract of the bacteria Escherichia coli ATCC 8739 with a concentration of 6% had antibacterial activity with weak inhibition, while the n-hexane fraction, ethyl acetate fraction and water fraction from rukam leaves had no antibacterial activity. In ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction did not have antibacterial activity against Staphylococcus aureus ATCC 6538. In ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction did not have antifungal activity. The antioxidant activity showed that the ethanol extract had strong antioxidant power, the n-hexane fraction had moderate antioxidant power, the ethyl acetate fraction and the water fraction had weak antioxidant power. The ethanol extract and n-hexane fraction did not have a toxicity effect, while the ethyl acetate and water fraction had a toxicity effect.
