Antioxidant enzyme capacity can be modulated by edible bird^s nest extract in H2O2-induced Spragues Dawley Rats Lina Elfita1*, Yardi1, Endang Suryani1, Nihayatul Mardliyah1, Muhammad Hudo Ardo1, Rinaldi1, Endah Wulandari2, Nurlaely Mida Rahmawati2, Chris Adyanto2
1 Department of Pharmacy, Faculty of Health Science, UIN Syarif Hidayatullah Jakarta
2 Faculty of Medicine, UIN Syarif Hidayatullah Jakarta
Abstract
Edible bird^s Nest (EBN) is a glutinous secretion from male swiftlets saliva. In this study, the effects of EBN extract on antioxidant capacity in Sprague Dawley (SD) rats induced by H2O2 were investigated. Groups of rats were treated with various dose of EBN extracts, and that effects on the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were examined. Thirty male SD rats were divided into 6 groups. Group 1 was treated with Na MCM. Group 2 and 3 were positive and negative control, which were treated with Vitamin E and distilled water, respectively. The other 3 groups were treated with different doses of EBN (10, 20 and 40 mg/kg bodyweight). The rats were treated for 30 days, and then were induced with H2O2 intraperitoneal at day 31 and 32, and all parameters were measured at day 33. CAT activity was significantly increased in group treated with 20 mg/kg bodyweight EBN extract by 33.3% and 9.5% before and after H2O2 induction, respectively. GPx activity increased 26.3% at day 30 in a group of rat treated with 40 mg/kg bodyweight EBN extract, however the GPx activity in this group was decreased 16.1% after H2O2 induction. GPx activity drastically decreased in control group as much as 45.1% after H2O2 induction. EBN extract did not show any effect on the SOD activity compared to control group. Thus, it is shown that EBN extract has capability to modulate antioxidant enzymes activity, therefore it potentially can be used n as antioxidant agent.
