Invitro Study of Bioactive Protein from Red Algae Symbiont Bacteria Eucheuma cottonii as Antibacterial and Anticancer Agent Siti Khairunnur(a), Ahyar Ahmad(a*), Rugaiyah Arfah(a), Hasnah Natsir(a), Nur Asmi(a), Desy Mardiana(a), Nabeela Nurvita(a)
Departement of Chemistry, Mathematics and Natural Science, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
*ahyarahmad[at]gmail.com
Abstract
Objective
This study aimed to determine antibacteria activity and toxicity of bioactive protein isolated from symbiotic bacteria of red algae Eucheuma cottonii.
Methods
Protein purification was done by fractination and dialysis, the results of purification were determined by the protein concentration using the Lowry method with Bovine Serum Albumin as standard. Antibacteria activity test by agar diffusion method, showed presence of bioactive protein compound that have inhibition ability towards the growth of pathogen bacteria such as Eschericia coli and Staphyllococcus aureus, and toxicity test by BSLT method, carried out by doing an examination of the toxicity level against Arthemia Salina.
Results
The results obtained showed that the symbiotic bacteria of red algae Eucheuma cottonii had the highest protein intracellular concentration in the fraction of 0-20% with strongest inhibition towards E.coli with 19.5 mm and fraction 20-40% towards S.aureus with inhibition area of 18.3 mm. Toxicity test showed intracellular protein fraction 0-20% have the highest toxicity with LC50 value of 18.298 ppm.
Conclusion
Symbiotic bacteria of red algae Eucheuma cottonii protein fraction has the potential to be developed as an antibacteria and anticancer agent in the future.
