EFFECT OF CALSIUM CHLORIDE ACTIVATOR ON TANSFORMING GROWTH FACTOR BETA LEVELS IN PLATELET RICH PLASMA Lisdiana Amin Asri1, Tenri Esa2, Uleng Bahrun2
1) Clinical Pathology Specialist Program, Faculty of Medicine, Hasanuddin University, Makassar. Jalan Perintis Kemerdekaan KM 10, Tamalanrea, Makassar, 90245. Indonesia.
Email: diana.aminasri[at]gmail.com
2) Department of Clinical Pathology, Faculty of Medicine, Hasanuddin University, Makassar. Jalan Perintis Kemerdekaan KM 10, Tamalanrea, Makassar, 90245. Indonesia.
Abstract
Objective: This study aimed to determine the levels of Transforming Growth Factor Beta (TGF Beta) in PRP performed using calcium chloride (CaCL2) as activator.
Method : This research used a laboratory experimental design, involving 16 healthy volunteers that met the inclusion and exclusion criteria.
Results: The study result showed that the mean pretreatment and PRP Acid Citrate Dextrose (ACD) platelet counts were significantly different (p<0.001). Platelet counts in PRP ACD were higher than pretreatment (666.06 compared to 326.44) or increase 104%. There was also significant difference (p<0.001) of TGF Beta levels between non activated and CaCl2 activated PRP ACD (17,05 ng/mL compared 53,13 ng/mL).
Conclusion: The levels of TGF Beta in PRP products activated with CaCl2 stored for 1 hour at room temperature (increased 212%). The levels of TGF Beta increased 3.12 times.
