SYNTHESIS OF GOLD NANOPARTICLES (AuNPs) WITH TYROSINE SUPPORTING AGENT AS COLORIMETRY SENSOR AGAINST Escherichia Coli BACTERIA Wahidah Febriya Ramadhani*, Abdul Karim*, Paulina Taba, Azmalaeni Rifkah A
*Department of Chemistry, Faculty of Math And Science
Hasanuddin University, Makassar-Indonesia
wahidahramadhani07[at]gmail.com/karimkimia[at]yahoo.com
Abstract
Abstract: Gold nanoparticles (AuNPs) can be applied as biosensors for Escherichia Coli bacteria. The sensory ability of AuNPs can be enhanced by the presence of a hood in the form of Tyrosine. At present, it has been investigated the synthesis of gold nanoparticles with 0.2 mM gold solution, reduced using a chemical method with ascorbic acid reducing agent in a mole ratio (1:3) resulting in the maximum wavelength absorption in UV-Vis Spectrophotometry is 525.40 nm and an absorbance of 0.833. After adding a covering agent in the form of Tyrosine, the concentration variation was 0.01 mM - 0.1 mM, resulting in a wavelength absorption range of 530 nm. Furthermore, the characterization of the nanoparticle bonds was tested by FTIR Spectrophotometry. Then it was applied as a sensor in a liquid media preparation of Escherichia Coli bacteria with a concentration of 10^8 CFU/mL - 10^6 CFU/mL, resulting in a wavelength absorption of batochromic shifts that marked the formation of bonds between AuNPs, Tyrosin. and Escherichia Coli bacteria.
Keywords: Gold Nanoparticles (AuNPs), Reducers of Ascorbic Acid, AuNPs-Tyrosine, and Escherichia Coli Bacteria.
