Cytotoxic Activity of Ethanol Extract Moringa Leave (Moringa oleifera) and Ethanol Extract Papaya Leave (Carica papaya) Combination on MCF-7 Breast Cancer Cells Nuni Rismayanti Nurkalbi1, Aryadi Arsyad2, Ika Yustisia3*
1. Graduated School, Hasanuddin University, Indonesia.
2. Departement of Physiology, Faculity of medicine, Hasanuddin University, Indonesia.
3. Departement of Biochemistry, Faculity of medicine, Hasanuddin University, Indonesia.
Abstract
Objective: This study aims to find out cytotoxic activities of the combination of ethanol extract moringa leave and ethanol extract papaya leave on the growth of MCF7 breast cancer cells culture.
Methods: This research was conducted in a true experimental laboratory using post test only control group design method.
Results: The results showed that there were similarities in the percentage of living cells in MCF7 cell culture using a combination of Moringa leaf ethanol extract and papaya leaf ethanol extract with concentrations of 0, 20, 40, 80 and 160 microgram/mL for 48 hours with a cell density of 5 x 103 after giving WST reagent and FITC v annexin kit reagent, namely a decrease in the number of living cells in several types of extract combinations and their concentrations. Inhibition of MCF7 cell culture was also indicated by the IC50 value which was included in the very strong category with details of each extract combination for 25:75 the IC50 value is 8.09 microgram/mL, for 50:50 the IC50 value is 12.02 microgram/mL, and for 75:25 the IC50 value is 24.40 microgram/mL. However, based on the results of the one way ANOVA test, there was no significant difference between groups (p more than 0.05).
Conclusion: The combination extract of ethanol extract moringa leave and ethanol extract papaya leave was inhibited the process of the MCF-7 cell culture growth showed by decrease the percentage of living cells.
