A natural product of anticancer peptides (ACPs) from symbiotic bacteria: Potential and Opportunities Nur Asmi (a), Ahyar Ahmad (a*), Hasnah Natsir (a), Muh. Nasrum Massi (b), Harishkumar Madhyastha (c), Radha Madhyastha (c), Yuichi Nakajima (c), Masugi Maruyama (c)
a) Department of Chemistry, Faculty of Mathematics and Natural Science, Hasanuddin University, Makassar 90245, Indonesia
*ahyarahmad[at]gmail.com
b) Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia
c) Department of Applied Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan
Abstract
Objective: This study was aimed to evaluate the bioactivity of the peptide from symbiotic bacteria at lung cancer LK-2 cell line compared normal M5S cell line
Methods: The separation of protein hydrolyzates based on molecular weight was carried out using a Molecular Weight Cut Off (MWCO) membrane to obtain peptide fragments. Peptide fragments ≤ 5 kDa, > 5-10 kDa, and > 10 kDa were tested for their anticancer activity using LK-2 lung cancer cells line compared to normal M5S cells line. The percentage of viable cells and cell proliferation were analyzed by MTT assay. The selectivity is determined using the Selectivity Index (SI) equation.
Result: The results showed the number of deaths of LK-2 cells increased when they were treated with peptide fragments at sizes ≤ 5 kDa, > 5-10 kDa, and > 10 kDa during the 16 hour treatment period. Peptide fragments resulted in the rate of cell proliferation were decreased at 16 h in the LK-2 cells, whereas the M5S cells did not show a significant decrease (P < 0.05) compared with control. The highest selectivity was shown by peptide fragment with a molecular weight ≤ 5 kDa and > 5-10 kDa; the value of SI is 3.
Conclusion: This discovery shows that peptide fragments that have sufficient selectivity are used as anticancer agents. This study shows the potential and opportunities of peptides from symbiont bacteria to be used as cancer therapy agents, especially lung cancer
Keywords: Symbiotic bacteria, peptide fragments, ACPs, lung cancer, selectivity index
