Purification, characterization, and anticancer assay of L-asparaginase enzyme from siam weed (Chromolaena odorata Linn) leaves against LK-2 lung cancer cells Yusriadi (a*,#), Ahyar Ahmad (a,b*,#), Rugaiyah A. Arfah (b), Hasnah Natsir (b), Nurul Khaerah (a), Nur Asmi (a), Nada Pratiwi Papriani (a), Marinda (a), Andi Fikrah Aulia Pamenta(a), Rafsanjany Ramadan (a), Harningsih Karim (c)
a) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Biochemistry Lab., Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
b) Laboratory of Research Centre and Developing of Sciences, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia
3) Department of Pharmacy, School of Pharmacy YAMASI, Mapala 2 Street Blok D5 No.10 Makassar 90222, Indonesia
*Email: ahyarahmad[at]gmail.com and yusri5077[at]gmail.com
#authors with equal contribution to this work
Abstract
Objective: L-asparaginase enzyme is known to be isolated from siam weed (C. odorata L.) leaves and also potentially to be developed as an anticancer treatment. This research aims to characterize, purify, and test the anticancer activity of the L-asparaginase enzyme krom C. odorata L. leaves against LK-2 cancer cells.
Methods: The enzyme activity was observed by Nessler Method, the enzyme purification was conducted by the chromatography techniques, and the cytotoxicity assay was observed by the MTT method.
Results: The optimum conditions of enzymes effectively at pH 8, 37oC and 30 minutes whereas the effect of the addition of metal ions namely K as the best activator able to increase 8.57% of enzyme activity while Zn as the best inhibitor capable of inhibiting 85.62% enzyme activity. The pure enzyme was obtained a specific activity of 11.25 IU/mg with 67.03 times of purity levels and the enzyme molecular weight of 11.8 kDa while the IC50 values were obtained at 100.3 ppm in the medium category.
Conclusion: These results indicate that the pure L-asparaginase enzyme isolated from C. odorata L. leaves has the potential to be expanded as an anticancer medicine, especially for LK-2 lung cancer.
Keywords: KEYWORDS : Anticancer, C. odorata L., characterization, LK-2, purification
