Identification of Bacteria and Evaluation of Frozen Semen Quality in Bali Bulls Using Different Extenders Vellani Septiya Putri (1), Trinil Susilawati (2), Anny Amaliya (3), Aulia Puspita Anugra Yekti (2*)
1)Student of animal Science Faculty, Brawijaya University, Malang, 65141, Indonesia
2)Singosari National Artificial Insemination Center, Singosari, Malang, 65153, Indonesia
3)Lecture of Animal Science Faculty, Brawijaya University, Malang, 65141, Indonesia
*Coresponding : auliapay[at]ub.ac.id
Abstract
Frozen semen plays an essential role in the success of artificial insemination in cattle, but its quality may decline due to bacterial contamination. The use of extenders with appropriate antibiotic combinations is necessary to maintain frozen semen quality and inhibit the growth of pathogenic bacteria. This study aimed to evaluate spermatozoa quality and identify pathogenic bacteria in frozen semen of Bali bulls using two different extenders, namely Tris aminomethane egg yolk and Trico with distinct antibiotic combinations. Frozen semen quality is a critical factor influencing the success rate of artificial insemination in cattle. Antibiotics in extenders play a crucial role in preserving sperm viability and preventing bacterial growth during freezing and thawing. In this study, semen samples were analyzed through laboratory trials involving two treatments with 10 replications each. Quality parameters observed included motility, viability, abnormalities, concentration, and total motile spermatozoa. Bacterial identification focused on four pathogenic species: Escherichia coli, Staphylococcus sp., Pseudomonas sp., and Mycoplasma sp. Descriptive analysis indicated no notable trends in semen quality between both extenders. Statistical comparison using independent t-test revealed no significant differences (P > 0.05) in any sperm quality parameters. Similarly, in bacterial counts between the Tris and Trico groups average E. coli bacteria was detected in Tris extenders (0.3x10^3 CFU/ml) and in Trico extenders were undetected. Average in Staphylococcus bacterias in Tris was (76.5x 10^3 CFU/ml) and in Trico (66.8x10^3 CFU/ml). Pseudomonas sp. were undetected. While Mycoplasma sp. bacteria average in Tris (76.6x 10^3 CFU/ml) and in Trico (95X10^3 CFU/ml). It can be concluded that differents extenders had no significant effect, but Trico extenders is a promising alternative to Tris.
