The Effect of Different Diluents on the Quality and Bacterial Content of Post-Thawing Frozen Semen of Madura Bulls Dodik Kristiawan (a), Trinil Susilawati (b), Anny Amalia (c), Aulia Puspita Anugra Yekti (b*)
a) Student of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
b) Lecturer of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
*Coresponding : auliapay[at]ub.ac.id
c) Singosari National Artificial Insemination Center, Singosari, Malang, 65153, Indonesia
Abstract
This study aimed to determine the effect of different extenders on the quality and bacterial content of post-thawing frozen semen of Madura cattle. The research material consisted of frozen semen Madura cattle produced by BBIB Singosari, Malang, comprising 10 straws using Tris aminomethane egg yolk extender and 10 straws using Trico egg yolk extender for microscopic quality testing, as well as 6 straws each for bacterial content analysis. The semen samples were collected from two Madura bulls aged 4 and 6 years with an average body weight of 535 kg. The study employed a laboratory experimental method with two treatments P0: frozen semen with Tris aminomethane egg yolk extender and P1: frozen semen with Trico egg yolk extender. Observed variables included individual motility, viability, abnormality, sperm concentration, total motile spermatozoa, and bacterial content (Escherichia coli, Pseudomonas sp., Staphylococcus sp., and Mycoplasma sp.). Data were analyzed using the paired t test (quality test) and mean analysis (bacterial content analysis). The results showed no significant differences (p>0.05) between the two treatments in semen quality and bacterial content. However, the Trico extender produced slightly better results, with motility of 45.3%, viability of 62.4%, abnormality of 6.2%, and total motile spermatozoa of 13.45 million/ml. E. coli and Pseudomonas sp. were undetected, while Mycoplasma sp. best values in Tris (34x103 CFU/ml) and Staphylococcus sp. best values in Trico (21,9x103 CFU/ml). It can be concluded that different extenders had no significant effect, but Trico egg yolk extender is a promising alternative to Tris.
