Differences in Incubation Time on the Quality of Holstein Friesian Cow Semen Sexing Method of Bovine Serum Albumin (BSA) Sedimentation 3 Gradients Dianita Ayu Puspitaloka (1), T. Susilawati (1), A. Amaliya (2), D. Sulistyowati (2), A.R.S. Mulyani (1), A.P.A. Yekti (1)*
Brawijaya University
Abstract
The gap between production and fulfillment of national animal protein requirements, especially milk, necessitates improvements in genetic engineering in the field of livestock reproduction through sperm sexing technology. The purpose of this study was to determine the effect of incubation time differences on the quality of sexed Friesian Holstein cattle semen using the 3-gradient BSA (Bovine Serum Albumin) sedimentation method. The research was conducted at the Singosari National Artificial Insemination Center Laboratory and the Faculty of Animal Science Livestock Reproduction Laboratory using fresh Friesian Holstein cattle semen collected at Singosari National Artificial Insemination Center with motility >70%. The method used in this study was a laboratory experiment with three incubation treatments (35, 40, and 45 minutes) and 10 replicates. Semen quality was evaluated microscopically and analyzed using ANOVA with a Randomized Block Design (RBD). The analysis results showed that there was no significant difference between incubation times on the microscopic quality of Friesian Holstein bull semen sexing using the BSA 3-gradient sedimentation method (P>0.05). The best average progressive motility value was in the upper fraction with an incubation time of 35 minutes at 66.33% and the lower fraction with an incubation time of 45 minutes at 56%. In conclusion, although there were descriptive differences in the figures, statistical tests showed that incubation time did not significantly affect the quality and proportion of Friesian Holstein sexed semen using the BSA 3-gradient sedimentation method.
Keywords: Keywords: Bovine Serum Albumin, Friesian Holstein, Incubation Time, Sedimentation, Spermatozoa Sex Determination
