Quality and Proportion of Frisian Holstein Sperm Using Egg White Albumin Sedimentation Sexing Method with Different Gradients
Andi Rezda Sri Mulyani1), Trinil Susilawati4), Anny Amalia3), Deny Sulistyowati3), and Hikma Nur Azizah2), Tsania syifatuzzahra2) and Aulia Puspita Anugra Yekti4*)

1)Graduate Student, Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.
2) Undergraduate Student, Department of Animal Science, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
3) Singosari National Artificial Insemination Center, Malang, 65153, Indonesia
4) Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
*Corresponding Author: auliapay[at]ub.ac.id

Abstract

This study aims to evaluate the effect of different gradients on the quality and proportion of sexed semen using the egg white albumin sedimentation method. This study was conducted at the Singosari Artificial Insemination Center in Malang, using Frisian Holstein semen with an average fresh motility of >70%. The study was conducted experimentally with 10 replicates and 2 treatments, namely T1 = egg white sedimentation sexing with 2 gradients (30% and 50%) and T2 = egg white sedimentation sexing with 3 gradients (30%, 40%, and 50%). The parameters observed included motility, viability, abnormality, membrane integrity, and the proportion of X and Y chromosomes. The quality evaluation results were analyzed using an independent t-test, while the determination of spermatozoa proportion was analyzed using Chi-Square. The results showed that the difference in gradients did not have a significant effect (P>0.05). on all quality parameters for each layer. In addition, the proportion of X and Y spermatozoa also showed no difference between the upper and lower fractions in both layers (P>0.05). In conclusion, spermatozoa separation using 3-gradient egg white albumin sedimentation provides more efficient separation and is able to maintain better spermatozoa quality compared to 2 gradients.

Keywords: Egg White Sedimentation, Frisian Holstein Bull, Sedimentation gradient, Semen Quality, and Spermatozoa Sexing

Topic: Animal Reproduction and Breeding