Artificial Insemination Success of Sexed Frozen Semen Using the Percoll Density Gradient Centrifugation Method in Frisien Holstein Crossbred Cattle at KAN Jabung
Aidil Agam (a*), Habib Asshidiq Syah (b), Aulia Puspita Anugrah Yekti (b)

Faculty Animal Science, Brawijaya University, Malang, 65145, Indonesia

Abstract

Friesian Holstein (FH) crossbred dairy cattle (PFH) play an important role in supporting national milk production in Indonesia- however, their productivity remains insufficient to meet domestic demand. One strategy to improve genetic quality and reproductive efficiency is through the application of artificial insemination (AI) technology. The development of semen sexing technology, particularly the Percoll Density Gradient Centrifugation (SGDP) method, enables the separation of X- and Y-chromosome-bearing spermatozoa with high accuracy, allowing the production of offspring with the desired sex-especially females for replacement stock in dairy farming. Nevertheless, sexed semen generally exhibits a lower conception rate compared to non-sexed semen, thus requiring evaluation under field conditions. This study aimed to evaluate the success of AI using non-sexed frozen semen and sexed SGDP semen in PFH cattle at Koperasi Agro Niaga (KAN) Jabung, Malang Regency. The study involved 2,158 cows divided into two treatment groups: 1,512 cows inseminated with non-sexed semen and 997 cows inseminated with sexed SGDP semen. The observed parameters included Conception Rate (CR), Pregnancy Rate (PR), and Service per Conception (S/C). Data were obtained from AI records, pregnancy checks, and farmer interviews, then analyzed descriptively. The results showed that non-sexed semen achieved CR and PR values of 18.06% and 41.27%, respectively, with an S/C value of 2.10. Meanwhile, sexed SGDP semen resulted in CR and PR values of 17.85% and 26.08%, with an S/C value of 1.40. These findings indicate that non-sexed semen produced a higher pregnancy rate, whereas sexed SGDP semen demonstrated greater reproductive efficiency by requiring fewer inseminations per conception.

Keywords: Artificial Insemination, Friesian Holstein, Sexed Semen, Percoll, Reproduction.

Topic: Animal Reproduction and Breeding