The Role of Indigofera-Based Feed with Tannin-Arginine on Testosterone Concentration, Plasma Protein, and SDS-PAGE Profile of Local Male Sheep Muhammad Fadhlirrahman Latief (a), (c), Osfar Sjofjan (b), Jasmal A. Syamsu (b), Tulus Maulana (d), Suyadi Suyadi (b)*
(a) Doctoral Program in Animal Science, Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Ketawanggede, Malang (65145).
(b) Faculty of Animal Science, Universitas Brawijaya, Jl. Veteran, Ketawanggede, Malang (65145).
* suyadi[at]ub.ac.id
(c) Faculty of Animal Science,Universitas Hasanuddin Jl. Perintis Kemerdekaan, Makassar (90245)
(d) Research Center for Applied Zoology, National Research and Innovation Agency Jl. Raya Bogor KM. 46, Cibinong, Bogor (16911)
Abstract
This study aimed to evaluate the effect of basal feeding of sorghum silage and pellet concentrate supplemented with tannin arginine on plasma testosterone concentrations, total protein content, and plasma protein profiles of local rams. Twelve rams (10 to 14 months old, weight 29 kg) were raised for 45 days at Balakosa Farm, Singosari, Malang, using a completely randomized design with three feeding treatments: concentrate without Indigofera (P0), low level Indigofera concentrate (P1), and high level Indigofera concentrate (P2), each with four replications. Plasma testosterone levels were analyzed using an ELISA method, while plasma protein concentrations were determined using the bicinchoninic acid assay at a wavelength of 562 nm. Protein profiles were analyzed using 1D SDS PAGE with a 4 to 20% gradient gel and visualized with Coomassie Brilliant Blue R 250 staining. The results showed that plasma testosterone levels were relatively stable across treatments, with values of 7.98 mg/dL (P0), 7.18 mg/dL (P1), and 7.65 mg/dL (P2). Plasma protein concentrations ranged from 174.41 to 175.80 microgram/mL and SDS PAGE analysis revealed a dominant protein band in the 15 to 120 kDa range, with relatively uniform band intensity across treatments, indicating no substantial changes in the plasma protein profile due to the administration of the Indigofera based diet with tannin arginine supplementation. These results indicate that Indigofera based feeds can be used without compromising male reproductive physiological performance, therefore potentially being applied in sustainable production systems.
Keywords: Indigofera, local sheep, plasma protein, testosterone, SDS-PAGE
