Kinetics of Fermented Cassava Stem Gas Production Using a Combination of Pleurotus ostreatus and Trichoderma asperellum as an Alternative Feed for Ruminants Agung Kusuma Wijaya1, Hartutik2*, Kusmartono2 and Mashudi2
1 Doctoral Student of Animal Science, Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, Indonesia
2 Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia.
* hartutik[at]ub.ac.id
Abstract
Cassava (Manihot esculenta) is an agricultural crop that produces a byproduct in the form of cassava stem waste, which is low in protein and high in crude fiber. Agricultural waste is increasingly being used in livestock farming as an alternative feed. This study aims to evaluate the effect of combining Pleurotus ostreatus and Trichoderma asperellum fungi on the gas production kinetics in Fermented Cassava Stems (FCS). It used a Completely Randomized Design (CRD) with a factorial setup of nine treatment combinations, each replicated three times. Cassava stems were fermented with P. ostreatus at 0%, 3%, and 6% and T. asperellum at 0%, 1.5%, and 3%. The in vitro gas production was conducted using sheep rumen fluid, with observations at 0, 2, 4, 8, 12, 24, 36, and 48 hours. After incubation, measurements included gas production, accumulation, and gas production kinetics. Results indicated no significant interaction (P>0.05) between P. ostreatus and T. asperellum in total gas production. The treatment without fungi produced the highest gas volume, 32.38 ml/500g at 24 hours and 48.65 ml/500g at 48 hours. Additionally, the combination of the two fungi showed no interaction (P>0.05) in gas production kinetics. It was concluded that fermented cassava stems without the addition of fungi can be used as a feed ingredient in complete sheep diets.
