Comparison of Microbial Counts in the Ileum of Native Chickens and Broiler Chickens Based on Total Plate Count (TPC).
Amara Brilliant Marcelline Alfariza1, Muhammad Halim Natsir2, Osfar Sjofjan2, Feri Eko Hermanto2, Yuli Frita Nuningtyas2*

1 Student of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
2 Lecturer of Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
*fritanuningtyas[at]ub.ac.id

Abstract

This study aims to compare the number of microbes in the ileum of native chickens and broiler chickens using the Total Plate Count (TPC) method. The research method used was a descriptive comparative approach. From each type of chicken, ileum samples were collected aseptically, then each sample was placed on 10 Petri dishes and mixed with de Mann Rogosa Sharpe (MRS) Agar using the pour plate method and incubated for 48 hours at 37C to count the number of bacterial colonies that grew. The data obtained were then analyzed using t test to determine significant differences between the two groups. The results showed that the number of microbes in the ileum of broiler chickens was significantly higher than that of native chickens with p value less than 0.05. This difference is likely due to factors such as the rearing system, diet, and growth differences between native chickens and intensively reared broiler chickens. The conclusion of this study indicates that there is a significant difference in the number of microbes in the ileum of native chickens and broiler chickens, suggesting that the rearing system and environmental factors have a significant impact on the composition of the intestinal microbiota.

Keywords: Broiler chickens, Native chickens, Ileum, Total Plate Count.

Topic: Animal feed and nutrition