Food security of goat meat in Surabaya city is based on the presence of Toxoplasma gondii
Hebert Adrianto1*, Victor Setiawan Tandean1, Billy Daniel Messakh1, Bracovanca Diwayestara Bravimasta2, Victor Kurniawan Yuwono3, and Firda Aulia Putri4

1 School of Medicine, Universitas Ciputra, Surabaya, Indonesia
2 Student of School of Medicine, Universitas Ciputra, Surabaya, Indonesia
3 Culinary Business, School of Tourism, Universitas Ciputra, Surabaya, Indonesia
4 Doctor, Universitas Ciputra graduate, Surabaya, Indonesia

Abstract

Toxoplasmosis is a human disease and an important zoonotic parasite caused by infection with Toxoplasma gondii through congenital or acquired routes. Consumption of goat meat containing cysts with bradyzoites is one of the main sources of T. gondii infection in humans and is a significant public health issue. This study aims to detect meat security based on the presence of T. gondii in goat meat. Goat meat samples were purchased at 10 supermarkets in West Surabaya with random sampling. 50 mg of goat meat was crushed and homogenized. Next, DNA was isolated from the homogenized tissue according to the ^pure-link genomic Isolation Kit^ procedure. T. gondii was detected via real-time polymerase chain reaction (PCR) from DNA extracted from meat. The results showed that PCR with primer BAG1 amplified T. gondii DNA in goat meat with a target of 470 bp. Two samples were positive for T. gondii DNA. Screening for meat consumption is very necessary before it reaches the public. Goat meat is cooked thoroughly.

Keywords: goat meat, Toxoplasma gondii, food, PCR, BAG1

Topic: Food Security and Food Processing