Identification of Colletotrichum sp. causing anthracnose disease on chilli pepper in 50 Kota District West Sumatera Indonesia
Riska1, Tri Budiyanti1, Jumjunidang 1, Ni Luh Putu Indriyani1, Sri Hadiati1, Nurmansyah1, Herwita Idris1, Ellina Mansyah1

Research Centre for Horticulture, National Research and Innovation Agency (BRIN). Jl. Raya Jakarta-Bogor Km 46, Cibinong, Bogor Indonesia 16911.

Abstract

Anthracnose fruit rot is an important disease of chilli pepper (Capsicum annuum L) in Indonesia. This study was carried out to identify and characterize the pathogens causing anthracnose disease in chilli peppers (C. annuum L.) grown in 50 Kota district, West Sumatera. Collection of symptomatic fruit were carried out in Luak 50 Kota district, and fungal pathogens were isolated on PDA media in vitro. One isolate chosen in this study is CM1. Morphological characterization, pathogenicity tests and amplification of DNA product with Internal Transcribed spacer region through polymerase chain reaction were demonstrated to identify and confirm that the isolate is the causal of fruit rot anthracnose. The result showed that CM1 isolate is grouped into Colletotrichum sp. isolate based on morphological characterization. Molecular identification and phylogenetic analyses of internal transcribed spacer regions showed that the CM1 isolate has high nucleotide identity with C. scovillei, with the percentage of homology with the reference isolate is 99.35 %. Pathogenicity testing showed that the isolate was pathogenic to red C. annuum both using mycelial plug and conidial suspension. A study on the identification and collection of C. scovillei isolate in Luak 50 Kota will be helpful to increase understanding the distribution of the pathogen in West Sumatera and essential for formulating the effective quarantine policy.

Keywords: Capsicum annum, antrachnose, West Sumatera

Topic: Food Security and Food Processing