The potential of Oryzias celebensis embryo heartbeat as a simple biomarker to detect the adverse effects of UV-C light
Regita Indriani Idris Khusnul Yaqin, Sri Wahyuni Rahim, Basse Siang Parawansa, Nita Rukminasari, Hadiratul Kudsiah, Rahmadi Tambaru, Dwi Kesuma Sari Etty Riani Andi Dina Hardiana Meimulya

Marine Science and Fisheries Faculty
Hasanuddin University

Abstract

The impact of radiation from UV rays is very important to observe on the environment and aquatic organisms. Research on the use of heartbeat as a simple biomarker in Oryzias celebensis embryos to detect the harmful effects of UVC has been carried out. This research was conducted using O.celebensis embryos exposed to UVC (250 nm) for 15, 10 and 5 minutes. The controls in this study were embryos without UVC irradiation. The embryo^s heart rate was calculated starting from stage 24 until hatching. ANOVA is used to determine differences in treatment rates between controls and treatments. The results showed that the UV exposure treatment of 15 minutes and 10 minutes was significantly different from the control (P<0.05). This proves that long UV exposure will have an impact on the embryo. The results of this study concluded that the heart rate of O.celebensis embryos can be used as a biomarker to detect the adverse effects of UVC.

Keywords: UV-C light, heartbeat, simple biomarker, embryo, Oryzias celebensis

Topic: Aquatic Pollution and Ecotoxicology