Field Trial of Vibrio Trivalent Inactivated Vaccine in Cantang Hybrid Grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) 1)Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Jl. Flora, Bulaksumur, Yogyakarta, Indonesia Abstract Vibriosis is a major bacterial disease in mariculture including marine fin fish worldwide. Cantang Hybrid Grouper (CHG) is the dominant fin fish species in Indonesian commercial mariculture, that is susceptible to vibriosis. The purposes of this study were to evaluate the effect of vibrio trivalent inactivated vaccine (VTIV) at the field trial level on the immune status, survival, and growth of CHG. A field trial experiment was conducted with two treatments namely vaccination and non-vaccination in two locations, PT Bali Barramundi and CV Maju Bersama Sejahtera, Gerokgak sub-district, Buleleng Regency, Bali. The VTIV is composed of three bacterial strains namely Vibrio harveyi SB25, Vibrio alginolyticus GD22, and Photobacterium damsela subspecies damsela GD05. Initial and booster vaccinations were conducted by intraperitoneal injection. The booster was carried out 14 days post the initial vaccination. Cantang grouper were reared in 3x3 m concrete tanks with a flow-through water system. Parameters observed were immune level, survival, growth rates, and feed conversion ratio (FCR). The results indicated that the titer antibody of vaccinated fish reached 16-fold higher than that of unvacinated fish. Vaccination also improved the phagocytic activity, phagocytic index, and respiratory burst of fish. The vaccinated CHG showed a higher survival rate with the highest survival rate of 96.91%, higher growth rate with the highest absolute growth of 120.14 g, the highest relative growth rate of 4.14%/day and the highest specific growth rate of 1.83%/day compared to the control treatment. Furthermore, the vaccinated CHG showed a more efficient FCR with the lowest FCR of 1.08. Field trial vaccination with VTIV in CHG significantly contributes to the improvement of the immune system. survival and growth performance of fish suggesting the urgent vaccination application in mariculture. Keywords: immune system, feed conversion ratio, growth, survival, Photobacterium Topic: Marine and Fisheries Biotechnology |
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