Methyltestosterone 17&#945-- Injection as A Safe Alternative Way to Enhance Maturation and Immune Response of Tiger Shrimp Penaeus monodon Transgenic F1
S Lante1, R Rosmiati1, A Parenrengi1, A Tenriulo1, E Suryati1

Research Center for Fishery, National Research and Innovation Agency, Indonesia

Abstract

Abstract: The present study investigated the effects of 17&#945--methyltestosterone on the reproductive performance and immune response of broodstock tiger shrimp Penaeus monodon transgenic F1. The reproduction, mature spawners, spermatophore weight and density, and immune response of total haemocyte count (THC) were evaluated. Males aged 13 months were divided into four groups. Groups 1,2 and 3 were injected 17&#945--methyltestosterone (17&#945--MT) at the dose of 100, 150, and 200 ng/100 g body weight three times with an interval of one week. Group 4 was eyestalk ablation. The specimen was kept in a concrete maturation tank for 10 tons. Then 14 days after the last injection (35th day), the males were harvested, weighed, and removed their spermatophores. THC was observed before, then on the 7th, 14th, and 35th days of the post-rearing period. 17&#945- -MT induction using dose-dependent increases the percentage of the mature spawner, spermatophore weight, spermatozoa density, and higher total hemocyte count (p>0.05) than eyestalk ablation. The THC injected with 17&#945--MT at three doses was higher (p<0.05) than ablation on the 7th and 14th days. Unlike on the 35th day, the highest THC was at a dose of 200 ng/100 g bw (p>0.05) among all treatments

Keywords: Immune response, reproductive performance, 17alpa methyltestosterone, tiger shrimp

Topic: Sustainable Aquaculture and Fisheries