Phytochemical Screening and Antioxidant Potency Test of Lindur Fruit Peel Extract (Bruguiera gymnorrhiza) Ana Mardliyah(a*), Naily Rizqiyah(b)
Universitas Islam Negeri Walisongo Semarang
Abstract
Degenerative diseases caused by free radicals can be treated with antioxidants, and the lindur plant (Bruguiera gymnorrhiza) is one of the mangroves that has the potential as an antioxidant. This research aims to determine the IC50 value of the antioxidant activity of lindur fruit peel extract and to analyze its secondary metabolite content. Extraction process of lindur fruit skin by maceration method. The yield from the maceration process of the crude methanol extract was 78.55% while that of the fractionation process from n-hexane, ethyl acetate and methanol-water were 1.2%, 1.52% and 65.24%, respectively. Secondary metabolites were analyzed by the phytochemical screening method. Based on the phytochemical test, the methanol crude extract contained alkaloids and saponins while the n-hexane fraction contained steroids, the ethyl acetate fraction contained alkaloids, and the methanol-water fraction contained saponins. Antioxidant activity was determined using the DPPH (1,1-diphenyl-2-picryhidrazil) method. The test results showed that for crude methanol extract the IC50 was 48.931 ppm and for the most active fraction was the ethyl acetate fraction the IC50 value was 73.040 ppm followed by the n-hexane fraction IC50 76.460 ppm, and the methanol-water fraction IC50 94.291 ppm.
