rbcL gene as a tool for DNA barcoding to identify marine diatom from Lampung Bay and Seribu island Elfira Rosa Pane, Yanti Rachmayanti, Zeily Nurachman
Program studi Doktor Kimia Institut Teknologi Bandung
Abstract
The focus of this study was to identify a region in the rbcL gene that can be utilized as a barcode to identify diatoms in the waters of Lampung Bay and the Thousand Islands. The methods employed included sampling, isolating, cultivating diatoms, examining diatom morphology, DNA isolation, polymerizing the rbcL and 18S genes, and strain classification through phylogenetic trees. Thirteen diatom strains were successfully grown and their DNA was isolated as a result of this investigation. Genetic identification based on the rbcL gene and 18S V4, followed by phylogenetic analysis, revealed that the SELBA1 strain belonged to the same clade as Serratifera takanoi- EPI1 strain belongs to the same lineage as Entomoneis sp species- ALBA1 strain belongs to the genus Actinocyclus- LLBA1 strain and Lauderia annulata belong to the same lineage- HLBA1 strain belongs to the same lineage as the Halamphora aponina strain- NLBA1 strain is classified as monophyletic with Halamphora coffeaeformis- TMA2 strain is classified in one clade with Cyclotella striata, NLA1 and PBI1 strains were classified with Navicula salinicola, NLBA2 strains were monophyletic with Navicula salinarum- TMGS1 strain was classified as one clade with Nitszchia inconspicua, PBI2 strain was classified as monophyletic with Nitszchia supralitorea, and TMA3 strain was classified as monophyletic with Psammodictyon panduriforme. There was a classification discrepancy between the two genes in the NLBA1 strain because the size of the 18S V4 gene in this strain was too short, hence the rbcL gene was utilized to identify this strain
Keywords: Diatom, Indonesian marine, rbcL gene, 18S V4, phylogenetic tree.
