Quantitative Determination of Thiol Functionalized Silica Particles by Ellman^s Reagent Malikhatun Ni^mah1, Muhammad Ali Zulfikar2, Zeily Nurachman1, Corresponding author: zeily@itb.ac.id
ITB
Abstract
To functionalize silica particles, tetraethoxysilane was reacted with 3-mercaptopropyl trimethoxysilane in basic condition to generate a golf ball-like particles that may be apply as functional materials for immunoglobulin G purification. Here, we quantitatively determined the amount of thiol group in a golf ball-like particles by the use of Ellman^s reagent. The functionalized silica particles were identified by the appearance of C-S binding vibration mode at wavelength number at 692 cm-1, Si-C vibration at 1340 cm-1, S-H vibration at 2550 cm-1, and at 2900 cm-1 for stretching of C-H of methylene. The abundance of S atom in modified nanoparticle silica observed by EDX was 11.22%. The concentration of thiol group in golf ball-like silica particles is 28.7 mmol/gram silica particles by the linear regression analysis of concentration thiol is y = 0.0118x + 0.003 with a correlation coefficient of 0.9993.
