Obtusinon A and B from the roots of Premna serratifolia Linn. (Lamiaceae) and their cytotoxicity against murine leukemia P-388 cells
Ajeng Dalli Alamiah Hadiati, Dikhi Firmansyah, Lia Dewi Juliawaty*

Organic Chemistry Division, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jalan Ganesha 10, Bandung, 40132, Indonesia.
*liadewi[at]itb.ac.id

Abstract

Premna serratifolia Linn. has been regarded as a beneficial healing herb in tropical Asia and East Africa and is listed as a traditional medicine to treat various diseases in many countries. In Indonesia, P. serratifolia Linn., known as buas-buas, grows a lot in the area of West Kalimantan and is used as traditional medicine and an edible plant. Two icetexane-type diterpenoids, namely obtusinon A and B, were isolated from the root extract of P. serratifolia. These two diterpenoids were isolated for the first time from the roots of P. serratifolia in Indonesia. Their structures were characterized based on 1D NMR spectroscopy data (1H NMR and 13C NMR), 2D NMR (HSQC and HMBC), FTIR, and UV-vis. These compounds were evaluated for cytotoxic activities against murine leukemia P-388 cells. Obtusinon A and B showed noticeable cytotoxic activity, with IC50 values of 0.91 and 0.28 microgram/mL, respectively. These finding indicated that icetexane-type diterpenoids have potency as anti-cancer and that the low-molecular-weight compounds have exceptional cytotoxic properties.

Keywords: Premna serratifolia- obtusinon A- obtusinon B- diterpenoids- cytotoxicity

Topic: CHEMISTRY AND MATERIAL SCIENCES