POTENTIAL OF METHANOTROPHIC BACTERIA NON-SYMBIOTIC NITROGEN FIXATION FROM MOROWALI REGENCY
Marhani (a*), Asmiaty Sahur (b), Adrianton (c), Jeki (d)

(a) Tadulako University
(b) Hasanudin University
(c) Tadulako University
(d) Tadulako University

Abstract

This study aims to identify the morphological, physiological methanotrophic bacteria, calculate. This research was conducted from September to December 2021 at the Laboratory of Biosciences and Plant Reproduction Biotechnology, Department Agricultural Cultivation, Faculty of Agriculture, Hasanuddin University. Soil sampling was carried out from April to October 2021 in the rhizosphere of irrigated lowland rice in the Witaponda, Bumiraya sub-districts and rainfed rice fields in Bungku Barat and Bungku Tengah Districts, Morowali Regency, Central Sulawesi Province and soil sample analysis was carried out at the Soil Chemistry and Fertility Laboratory Department of Soil Science, Faculty of Agriculture, Hasanuddin University The results of the research from 27 isolates identified were selected 11 isolates. Based on the gram reaction test, 11 gram negative isolates were selected. 11 isolates were anaerobic. Based on the catalase test, 11 isolates were catalase positive, 11 isolates were capable of nitrogen fixation, five isolates were yellowish white, five isolates were white, one isolate was yellow, six isolates had convex elevation, and five isolates had elevation which is flat Based on the yield characteristics of the Witaponda sub-district, five isolates with isolate codes WPM1, WPM2, WPM3, WPM4, WPM5 were found. Bumiraya sub-district as many as three isolates with isolate codes BRM1, BRM2, BRM3. West Bungku District produced two isolates with the isolate code BBM1, BBM2 and Bungku Tengah District as much as one isolate with the code BTM1. Based on the results of the calculation of the number of soil bacteria found in the Witaponda subdistrict as much as 54.05 times 106 Cfu per mL in Bumiraya subdistrict as much as 40.21 times 106, Cfu per mL in West Bungku subdistrict as much as 12.06 times 106 Cfu per mL in Bungku Tengah subdistrict as much as 11.65 times 106 Cfu per mL. The results of the analysis of chemical properties show that pH, CEC, organic C, P205, K, are classified as low in both irrigated and rainfed rice fields.

Keywords: Rice, Methanotrophic Bacteria, Nitrogen Fixation

Topic: Agriculture Productivity