Determination of Antimicrobial Resistance Profile of Salmonella Enteritidis isolated from chicken and human in Indonesia
Tati Ariyanti*, Suhaemi, Sri Mulyati, Faidah Rachmawati, Susan M Noor

Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia

Email: tati011[at]brin.go.id*

Abstract

Salmonella enteritidis is a foodborne pathogen that can be transmitted to humans through direct contact with infected livestock or contaminated chicken food. Antibiotic overuse as a therapy in both animals and humans can result in antimicrobial resistance in bacteria. This study determined the profile of antibiotic resistance. A total of 52 S. enteritidis isolates were tested for antibiotic susceptibility in the Bacteriology Laboratory of the National Research Center for Veterinary Research, BRIN. These isolates came from chickens (43 isolates), farm water (2 isolates), and humans (7 isolates). The agar diffusion technique was used to conduct the Antimicrobial Sensitivity Test (AST) (Kirby-Bauer). Salmonella enteritidis was resistant to 11 antibiotics, including Colistine Sulfate (75%), Meropenem (67.31%), Nalidixic acid (67.31%), Cefotaxime (65.38%), Ampicillin (61.54%), Ceftazidime (50%), Streptomycin (26.92%), Gentamycin (15.38%), Tetracycline (9.62%), Chloramphenicol (5.77%), Ciprofloxacin (1,92%). Salmonella enteritidis isolates were found to be multi-drug resistant to a variety of antibiotics, with 76.92% of isolates resistant to 1-5 antibiotics and 23.08% resistant to 6-10 antibiotics. These findings suggest that local isolates of S. enteritidis in chickens and humans in this study were resistant to these antibiotics, which could be due to the use of antibiotics for therapy or the spread of resistant strains.

Keywords: Salmonella enteritidis, resistance, AST, chickens, humans.

Topic: Agricultural Socio-Economics