Serologic Test Agreement in Diagnose Mycoplasma gallisepticum (MG) in Commercial Layers
Hastuti Handayani S Purba1*, Faidah Rachmawati2, Eko S Purwanto2, Rida Tiffarent3, Tati Ariyanti2, Susanti4, Muhammad I Desem4, Riyandini Putri2, Zul Azmi5, Sri Suryatmi Prihandini2, Didik T Subekti6, Susan M Noor2

1 Research Center for Genetic Engineering, National Research and Innovation Agency. Bogor 16911, West Jawa Province, Indonesia
2 Research Center for Veterinary Science, Research Organization for Health, National Research and Innovation Agency. Bogor 16911, West Jawa Province, Indonesia
3 Research Center for Applied Zoology, National Research and Innovation Agency. Bogor, 16911, Indonesia
4 Bureau for Organization and Human Resources, National Research and Innovation Agency. Jakarta, 10340, Indonesia
5 Goats Research Station, PO Box 1 Galang, Sei Putih 20585 - North Sumatra, Indonesia
6 Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency. Bogor 16911, West Jawa Province, Indonesia

Email:hast008[at]brin.go.id*

Abstract

Mycoplasma gallisepticum (MG) infection in chickens results in large economic losses because it significantly reduces egg production. Chickens that have been infected with MG are stated to have a higher susceptibility to other disease agents. Therefore it is important to monitor the presence of MG in livestock. Monitoring through serological tests is the easiest option to apply. Rapid Agglutination Serum (RSA) is an option because of its fast and inexpensive application. In addition, ELISA has also been used to confirm the RSA test. This study aims to compare the suitability of two serological tests, namely the RSA and ELISA. Serum samples of 155 taken from laying hens were tested by RSA and ELISA. RSA test results obtained 99 (63.9%) seropositive and 105 (67.7%) ELISA seropositive. As many as 68 (64.8%) were seropositive on the RSA and ELISA tests, while 19 (38%) were seronegative. The proportion of conformity by McNemar test between the RSA and ELISA tests did not show a significant difference of 0.545 (P>0.005). The kappa value between the two serological techniques was low (k=0.027). The statistical analysis results of these two serological tests show that these two tests can be used as initial monitoring for screening for MG.

Keywords: Mycoplasma gallisepticum, Serologic Monitoring, RSA, ELISA

Topic: Agriculture Productivity