Novel Polymorphism of p.R25I in the Bovine PRM1 Gene and Its Association with Sperm Quality Traits of Indigenous Donggala Bulls (Bos indicus) (a) Research Center for Applied Zoology, National Research and Innovation Agency (BRIN), Cibinong, Bogor, West Java, Indonesia 16911 Abstract Donggala cattle (Bos indicus) are one of Indonesian native cattle from Central Sulawesi that kept for meat production purpose. This study aimed to observe the genetic diversity of Protamine 1 (PRM1) gene of Donggala bulls using a sequencing method. A total six Donggala bulls from the breeding station (UPTD Pembibitan Sapi Potong Provinsi Sulawesi Tengah) were used in the present study. The results showed that a novel missence mutation of p.R25I (Arginine →- Isoleucine) was detected in the PRM1 gene (exon 1) of Donggala bulls. In this study, two bulls have the heterozygous RI genotype and the another bulls have a normal (RR) genotype. While, the mutant genotype (II) was absence in the animals study. However, the many sperm qualities of volume, motility and concentration in RR bulls were higher than RI bulls but not different significantly. In conclusion, the I allele may be had the negative impact on sperm quality of Donggala bulls. Hence, the further study with large samples is important to confirm this finding accurately. Keywords: Central Sulawesi- Donggala bulls- Mutation- PRM1 gene- Sperm quality Topic: Animal Genetics, Breeding and Livestock Production |
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